PJB-2009-152
PRODUCTION AND PURIFICATION OF CELLULOSE-DEGRADING ENZYMES FROM A FILAMENTOUS FUNGUS TRICHODERMA HARZIANUM
SIBTAIN AHMED, AMMARA BASHIR, HUMA SALEEM, MUBSHARA SAADIA AND AMER JAMIL*
Abstract
The major components of plant cell walls are cellulose, hemicellulose and lignin, with cellulose being the most abundant component. Cellulose degrading-enzymes provide a key opportunity for achieving tremendous benefits of biomass utilization. Trichoderma harzianum, a filamentous fungus, produces cellulose degrading enzymes and has been exploited by the industry. In this study, three cellulases, exoglucanase (EXG), endoglucanase (EG) and b-glucosidase (BGL) were partially purified from T. harzianum. The optimal pH, temperature and incubation time for cellulases production was found to be 5.5
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